在登革热病毒期间监测白纹伊蚊C6/36 细胞系的线粒体功能感染

Monitoring mitochondrial function in Aedes albopictus C6/36 cell line during dengue virus infection

Keywords：Aedes aegypti, Dengue virus, Mitochondria

关键词：埃及伊蚊、登革热病毒、线粒体
作者：Santana-Román ME, Maycotte P, Uribe-Carvajal S, Uribe-Alvarez C, Alvarado-Medina N, Khan M, Siddiqui A, Pando-Robles V 
出版期刊：《Insects》（2021）

Abstract：

Aedes aegypti and Aedes albopictus mosquitoes are responsible for dengue virus (DENV) transmission in tropical and subtropical areas worldwide, where an estimated 3 billion people live at risk of DENV exposure. DENV-infected individuals show symptoms ranging from sub-clinical or mild to hemorrhagic fever. Infected mosquitoes do not show detectable signs of disease, even though the virus maintains a lifelong persistent infection. The interactions between viruses and host mitochondria are crucial for virus replication and pathogenicity. DENV infection in vertebrate cells modulates mitochondrial function and dynamics to facilitate viral proliferation. Here, we describe that DENV also regulates mitochondrial function and morphology in infected C6/36 mosquito cells (derived from Aedes albopictus). Our results showed that DENV infection increased ROS (reactive oxygen species) production, modulated mitochondrial transmembrane potential and induced changes in mitochondrial respiration. Furthermore, we offer the first evidence that DENV causes translocation of mitofusins to mitochondria in the C6/36 mosquito cell line. Another protein Drp-1 (Dynamin-related protein 1) did not localize to mitochondria in DENV-infected cells. This observation therefore ruled out the possibility that the abovementioned alterations in mitochondrial function are associated with mitochondrial fission. In summary, this report provides some key insights into the virus-mitochondria crosstalk in DENV infected mosquito cells.

文章摘要：

埃及伊蚊和白纹伊蚊是登革热病毒 (DENV) 在全球热带和亚热带地区传播的罪魁祸首，估计有 30 亿人生活在接触 DENV 的风险中。感染 DENV 的个体表现出从亚临床或轻度到出血热的症状。受感染的蚊子不会显示出可检测到的疾病迹象，即使病毒会保持终生持续感染。病毒和宿主线粒体之间的相互作用对于病毒复制和致病性至关重要。脊椎动物细胞中的 DENV 感染调节线粒体功能和动力学以促进病毒增殖。在这里，我们描述了 DENV 还调节受感染的 C6/36 蚊子细胞（源自白纹伊蚊）中的线粒体功能和形态。我们的研究结果表明，DENV 感染增加了 ROS（活性氧）的产生，调节了线粒体跨膜电位并诱导了线粒体呼吸的变化。此外，我们提供了第一个证据表明 DENV 导致 C6/36 蚊子细胞系中有丝分裂素易位至线粒体。另一种蛋白 Drp-1（Dynamin 相关蛋白 1）未定位于 DENV 感染细胞中的线粒体。因此，这一观察结果排除了上述线粒体功能改变与线粒体裂变有关的可能性。总之，本报告提供了一些关于 DENV 感染蚊子细胞中病毒-线粒体串扰的关键见解。

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