伊蚊细胞系作为研究蚊子-沃尔巴克氏体共生体代谢和免疫相互作用的新工具

Aedes fluviatilis cell lines as new tools to study metabolic and immune interactions in mosquito-Wolbachia symbiosis

 

 

 

Keywords:Energy metabolism regulation; immune response; host metabolism; immune pathway activation
关键词:能量代谢调节;免疫反应;宿主代谢;免疫通路激活

非哺乳动物:软体动物
作者:Conceição CC, da Silva JN, Arcanjo A, Nogueira CL, de Abreu LA, de Oliveira PL, Gondim KC, Moraes B, de Carvalho SS, da Silva RM, da Silva Vaz I Jr, Moreira LA, Logullo C
出版期刊:《Scientific Reports》 2021/9/28


Abstract:

In the present work, we established two novel embryonic cell lines from the mosquito Aedes fluviatilis containing or not the naturally occurring symbiont bacteria Wolbachia, which were called wAflu1 and Aflu2, respectively. We also obtained wAflu1 without Wolbachia after tetracycline treatment, named wAflu1.tet. Morphofunctional characterization was performed to help elucidate the symbiont-host interaction in the context of energy metabolism regulation and molecular mechanisms of the immune responses involved. The presence of Wolbachia pipientis improves energy performance in A. fluviatilis cells; it affects the regulation of key energy sources such as lipids, proteins, and carbohydrates, making the distribution of actin more peripheral and with extensions that come into contact with neighboring cells. Additionally, innate immunity mechanisms were activated, showing that the wAflu1 and wAflu1.tet cells are responsive after the stimulus using Gram negative bacteria. Therefore, this work confirms the natural, mutually co-regulating symbiotic relationship between W. pipientis and A. fluviatilis, modulating the host metabolism and immune pathway activation. The results presented here add important resources to the current knowledge of Wolbachia-arthropod interactions.

 

文章摘要:

在目前的工作中,我们从蚊子 Aedes fluviatilis 中建立了两种新的胚胎细胞系,它们含有或不含天然存在的共生细菌 Wolbachia,分别称为 wAflu1 和 Aflu2。在四环素处理后,我们还获得了没有 Wolbachia 的 wAflu1,命名为 wAflu1.tet。进行形态功能表征以帮助阐明在能量代谢调节和所涉及的免疫反应的分子机制的背景下的共生体-宿主相互作用。 Wolbachia pipientis 的存在提高了 A. fluviatilis 细胞的能量性能;它影响对关键能量来源(如脂质、蛋白质和碳水化合物)的调节,使肌动蛋白的分布更加外围,并具有与邻近细胞接触的延伸部分。此外,先天免疫机制被激活,表明 wAflu1 和 wAflu1.tet 细胞在使用革兰氏阴性菌刺激后有反应。因此,这项工作证实了 W. pipientis 和 A. fluviatilis 之间的自然、相互调节的共生关系,调节宿主代谢和免疫通路激活。此处介绍的结果为当前有关沃尔巴克氏体-节肢动物相互作用的知识增加了重要资源。

 


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